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Merck KGaA
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Cloud-Clone corp
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Enzyme Immunoassay for the Quantitative Determination of Rat CXC-Chemokine ligand 12, CXCL12 in serum, plasma, tissues and other biological samples
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Image Search Results
Journal: Oncotarget
Article Title: Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study
doi: 10.18632/oncotarget.9949
Figure Lengend Snippet: ( A ) CXCL12 plasma levels of MF patients (total n =24; JAK2 V617F mutated patients n = 16; CALR mutated patients n = 8) and controls ( n = 10). Regardless mutation status, CXCL12 concentration was significantly higher in MF patients ( *p ≤ 0.05 vs controls ). ( B ) When cells were migrated toward CXCL12 alone, an increased migration ability was observed in MF-derived ( n = 15) CD34 + cells as compared with the CB-derived ( n = 8) counterparts. The addition of inflammatory factors alone (IL-1β/TNF-α) plus CXCL12 significantly increased the migratory behaviour of MF-derived CD34 + cells as compared with CXCL12 alone. IL-1β + TNF-α synergistically enhanced the migratory behaviour of CD34 + cells as compared with spontaneous migration ( ****p < 0.0001 ), CXCL12 alone ( **p <0.001 ) and the CB-counterpart ( #### p <0.0001). Results are expressed as mean percentages ± SEM of input. (**p ≤ 0.01; ***p ≤ 0.001 vs CXCL12 alone for CB-derived CD34 + cells ) ( *p ≤ 0.05; **p ≤ 0.01; ****p < 0.0001 vs spontaneous migration for MF-derived CD34 + cells ) ( # p ≤ 0.05; #### p <0.0001 vs CB ).
Article Snippet: In particular, the TIMP-1 ELISA kit was provided from Boster Immunoleader (Boster Biological Technology Co., Pleasanton, CA, USA) and
Techniques: Mutagenesis, Concentration Assay, Migration, Derivative Assay
Journal: Oncotarget
Article Title: Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study
doi: 10.18632/oncotarget.9949
Figure Lengend Snippet: Panels ( A and B ) show the clonogenic potential of CB-derived (A; n = 6) and MF-derived CD34 + cells (B; n = 14) at baseline with or without various combinations of pro-inflammatory factors (CFU-C) and after migration toward CXCL12 alone or various combinations of pro-inflammatory factors + CXCL12 (CFU-C post-migration). After migration toward IL-1β + TNF-α + CXCL12 ± TIMP-1, the MF-derived, but not CB-derived, CD34 + cells show significantly increased clonogenic potential. Results are expressed as mean fold change of CFU-C ± SEM. ( *p ≤ 0.05 vs untreated cells (A) and CXCL12 alone (B) ).
Article Snippet: In particular, the TIMP-1 ELISA kit was provided from Boster Immunoleader (Boster Biological Technology Co., Pleasanton, CA, USA) and
Techniques: Derivative Assay, Migration
Journal: PLoS ONE
Article Title: Microdialysis Sampling from Wound Fluids Enables Quantitative Assessment of Cytokines, Proteins, and Metabolites Reveals Bone Defect-Specific Molecular Profiles
doi: 10.1371/journal.pone.0159580
Figure Lengend Snippet: List of used enzyme-linked immunosorbent assay (ELISA) kits. All kits were purchased as indicated and used according to the manufacture’s guide.
Article Snippet: SDF-1 (CXCL12) ,
Techniques: Enzyme-linked Immunosorbent Assay
Journal: PLoS ONE
Article Title: Microdialysis Sampling from Wound Fluids Enables Quantitative Assessment of Cytokines, Proteins, and Metabolites Reveals Bone Defect-Specific Molecular Profiles
doi: 10.1371/journal.pone.0159580
Figure Lengend Snippet: Samples were analyzed by ELISA. Values are mean ± SD. IL-6, interleukin-6; CXCL2, chemokine (C-X-C motif) ligand 2; CXCL7, chemokine (C-X-C motif) ligand 7; CXCL3, chemokine (C-X-C motif) ligand 3; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL4, chemokine (C-X-C motif) ligand 4; IL-10, inteleukin-10; TGF-β1, transforming growth factor-β1; SDF-1, stromal-cell derived factor-1. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: SDF-1 (CXCL12) ,
Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay